In vitro cytotoxicity and genotoxicity screening of Cuscuta arvensis Beyr. and Achillea wilhelmsii C. Koch in preclinical analysis for further drug design

Plant-based compounds have been used for medicinal purposes since ancient times, as easily accessible and low-cost treatment options. Despite the widespread belief that plants are quite safe devoid of side effects, scientific studies revealed toxicity potential active components on healthy cells. The present study was designed to investigate in vitro cytotoxicity genotoxicity Achillea wilhelmsii C. Koch Cuscuta arvensis Beyr., which frequently traditional medicine. In this context, evaluation extracts performed by MTT (3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. Our results indicated extract from A. did not affect viability fibroblasts at any concentrations, but rather significantly stimulated cell proliferation a concentration 25 µg/mL. On other hand, reduced all concentrations tested. second part research, DNA damaging investigated comet assay non-cytotoxic concentrations. caused significant increase percentage tail (%TDNA), is considered an indicator damage, only highest concentration, while %TDNA methanolic may be up 100 μg/mL, however, factor limiting its use.